Application

Research Sub CategoryNeuronal & Glial Markers

Research CategoryNeuroscience

Immunocytochemistry

Immunohistochemistry: 1:30

Optimal working dilutions must be determined by end user.

Anti-Neuron Cell Surface Antigen Antibody, clone A2B5-105 (7), Alexa Fluor 488 detects level of Neuron Cell Surface Antigen & has been published & validated for use in IC, IH.

Disclaimer

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Embryonic chicken retinal cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin conjugated to Alexa Fluor^® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Specificity

The antibody recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:

⋅ Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)

⋅ Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979.

⋅ Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989].

⋅ Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).

⋅ Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).

⋅ Human and rat thymic epithelial cells (Haynes, et al., 1988).

⋅ Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & slerberg, 1989).

⋅ Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).

Storage and Stability

Maintain at 2-8°C in undiluted aliquots in the dark for up to 6 months after date of receipt.